antibodies against anti itga4  (Cusabio)

Journal: bioRxiv

Article Title: NSD2 promotes cell durotaxis and drives the transition from PKD to tubulocystic renal cell carcinoma through integrin/FAK/AKT signaling

doi: 10.1101/2024.10.21.619559

Figure Lengend Snippet: (A) Normalized read density of H3K36me2 CUT&Tag signals of Ksp Cre ; Nsd2 OE/+ and Nsd2 OE/+ mice at 12 weeks from 3 kb upstream of the TSS to 3 kb downstream of the TES. (B) KEGG pathway analysis of gene expression changes in CUT&Tag data. (C) Venn diagram showing the number of genes harboring H3K36me2 binding and the differential expression genes determined by RNA-seq data. (D) The KEGG pathway analysis of the overlapping genes in (C). (E) RT-qPCR analysis of integrin family mRNA levels of KM and KMN mice (n = 3 per group). Experiments were repeated at least three times, with similar results. (F) RT-qPCR analysis of ITGA4 and ITGA11 mRNA levels of EV and Nsd2-OE cells (Caki-1 and 293T cells derived, n = 3 per group). Experiments were repeated at least three times, with similar results. (G) RT-qPCR analysis of ITGA4 and ITGA11 mRNA levels of Ctrl and Nsd2-KO cells (Caki-1 and 293T cells derived, n = 3 per group). Experiments were repeated at least three times, with similar results. (H) IHC staining and quantification of ITGA4 and ITGA11 expressions in KM and KMN mice at 12 weeks (n = 5 per group). Experiments were repeated at least three times, with similar results, and representative images are shown. Scale bars: 50 μm. (I) Snapshot of H3K36me2 CUT&Tag signals at the Itga4 and Itga11 gene in Nsd2 OE/+ and Ksp Cre ; Nsd2 OE/+ mice. (J) ChIP-qPCR of Itga4 and Itga11 in Nsd2 OE/+ and Ksp Cre ; Nsd2 OE/+ mice. The location of the ChIP primer pairs used is at the promoter area. Experiments were repeated at least three times, with similar results. (K) The Pearson product-moment pair-wise gene correlation analysis between NSD2 and ITGA4 , ITGA11 with TCGA database.

Article Snippet: Next, samples were blocked with 5% BSA at room temperature for 1 h. Then, samples were incubated with primary antibodies at 4 °C for 12-16 h. The primary antibodies used were NSD2 (Abcam, ab75359), H3K36me2 (Abcam, ab9049), AMACR (Abcam, ab140798), Vimentin (Cell Signaling Technology, 5741), Ki67 (Abcam, ab15580), CK19 (Abcam, ab15463), Collagen I (Cell Signaling Technology, 72026), ITGA11 (Abcam, ab198826), ITGA4 (Cell Signaling Technology, 8440), p-FAK (Cell Signaling Technology, 3283), p-AKT (Cell Signaling Technology, 4060), Collagen V (Abcam, ab7046).

Techniques: Gene Expression, Binding Assay, Quantitative Proteomics, RNA Sequencing, Quantitative RT-PCR, Derivative Assay, Immunohistochemistry, ChIP-qPCR

Journal: bioRxiv

Article Title: NSD2 promotes cell durotaxis and drives the transition from PKD to tubulocystic renal cell carcinoma through integrin/FAK/AKT signaling

doi: 10.1101/2024.10.21.619559

Figure Lengend Snippet: (A) Scheme of treatment given for each gavage. (B) Kidney images of KMN mice treated with vehicle or Firategrast for 21 days. Quantification of kidney volume (mm 3 ) is on the right (n = 6 per group). (C) Representative hematoxylin and eosin images of kidney tissue from KMN mice treated with vehicle or Firategrast for 21 days. Scale bars: 100 μm. Quantification of cystic index (%) and number of cysts per field is shown at the bottom (n = 6 per group). (D) IF analysis of Collagen I expression and IHC staining of p-FAK, ITGA4, and ITGA11 in KMN mice treated with vehicle or Firategrast for 21 days. Scale bars: 50 μm. Quantification of Collagen I, p-FAK, ITGA4 and ITGA11 positive area is shown at the bottom (n = 6 per group). (E) IHC staining and quantification of CK19, AMACR, Vimentin positive area, and Ki67 positive cells per field from indicated mice (n = 6 per group). Scale bars: 50 μm. (F) CCK8 assay of EV and Nsd2-OE cells transfected with the indicated plasmid, respectively. Experiments were repeated at least three times, with similar results. (G) CCK8 assay of EV and Nsd2-OE cells treated with MK2206 or vehicle, respectively. Experiments were repeated at least three times, with similar results.

Article Snippet: Next, samples were blocked with 5% BSA at room temperature for 1 h. Then, samples were incubated with primary antibodies at 4 °C for 12-16 h. The primary antibodies used were NSD2 (Abcam, ab75359), H3K36me2 (Abcam, ab9049), AMACR (Abcam, ab140798), Vimentin (Cell Signaling Technology, 5741), Ki67 (Abcam, ab15580), CK19 (Abcam, ab15463), Collagen I (Cell Signaling Technology, 72026), ITGA11 (Abcam, ab198826), ITGA4 (Cell Signaling Technology, 8440), p-FAK (Cell Signaling Technology, 3283), p-AKT (Cell Signaling Technology, 4060), Collagen V (Abcam, ab7046).

Techniques: Expressing, Immunohistochemistry, CCK-8 Assay, Transfection, Plasmid Preparation

Journal: eLife

Article Title: Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

doi: 10.7554/eLife.99172

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-ITGB1, ITGB4, ITGA4 , Cell Signaling , #4749 , WB (1/1000).

Techniques: Transfection, Construct, Expressing, Plasmid Preparation, Marker, Sequencing, Purification, Transduction, Control, Software, Staining

Journal: International Journal of Molecular Sciences

Article Title: Canonical and Non-Canonical Localization of Tight Junction Proteins during Early Murine Cranial Development

doi: 10.3390/ijms25031426

Figure Lengend Snippet: ZO-1 shows the strongest expression on the apical surface of the mouse neuroepithelium. ( A ): Confocal images show a representative coronal section of mouse cranial neural folds with immunofluorescence co-staining detecting ZO-1 (yellow) and integrin alpha 4 (ITGA4, magenta) at embryonic stage E 8.5 (9 somites, 9s). The overlay image of all channels (merge) is shown at the top of panel ( A ), followed below by the individual channel images, showing the signals for ITGA4 (magenta) and ZO-1 (yellow). Nuclei are stained with DAPI (cyan). Scale bar: 50 µm. Immunofluorescence staining for ITGA4 was performed to label the basolateral cellular domains. ZO-1 showed the strongest signals in the neuroectoderm (NE) and less strong signals in the non-neuronal ectoderm (NNE) and the mesenchymal cells, underlying the neuronal ectoderm. ( A′ ): Higher magnification of boxed area in ( A ). The overlay image of all channels is shown at the top of panel ( A′ ), followed below by the individual channel image, showing the signals for ZO-1 (yellow). Scale bar: 5 µm. ZO-1 was localized to the apical cell–cell junctions. ( B ): Confocal images show representative coronal section of mouse cranial neural folds with immunofluorescence co-staining detecting ZO-1 (yellow) and ITGA4 (magenta) at embryonic stage E 9.0 (13 somites, 13s). The overlay image of all channels is shown at the left of panel ( B ), followed to the right by the individual channel images, showing the signals for ITGA4 (magenta) and ZO-1 (yellow). Nuclei are stained with DAPI (cyan). Scale bar: 50 µm. ZO-1 showed the strongest signals in the neuroectoderm (NE) and less strong signals in the non-neuronal ectoderm (NNE) and mesenchymal cells. ( C ): E 8.0 whole-mount mouse embryos (6 somites, 6s) were immunofluorescence co-labelled for ZO-1 (yellow) and ITGA4 (magenta). The overlay image of both channels is shown at the top of panel ( C ), followed below by the individual channel image showing the signals for ZO-1 (yellow). The frontal view on the whole-mount forebrains, imaged using confocal microscopy, is shown. Scale bar: 50 µm. ( C′ ): Magnification of boxed area indicated in ( C ) with merged-channel image at the top, showing ITGA4 (magenta) and ZO-1 (yellow) signals and ZO-1 single-channel image below. Scale bar: 10 µm.

Article Snippet: Rabbit ITGA4 , Cusabio, Houston TX, USA, CSB-PA011867LA01HU , 1:200.

Techniques: Expressing, Immunofluorescence, Staining, Confocal Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Canonical and Non-Canonical Localization of Tight Junction Proteins during Early Murine Cranial Development

doi: 10.3390/ijms25031426

Figure Lengend Snippet: List of antibodies.

Article Snippet: Rabbit ITGA4 , Cusabio, Houston TX, USA, CSB-PA011867LA01HU , 1:200.

Techniques: